Government Title Research Associate III- Natural Products Categories Government Organization Frederick National Laboratory for Cancer Research (Leidos Biomed) Location Frederick, Maryland Website https://frederick.cancer.gov/ Contact email@example.com Job Information Link to apply: https://leidosbiomed.csod.com/ats/careersite/jobdetails.aspx?site=4&c=leidosbiomed&id=305 The Frederick National Laboratory is dedicated to improving human health through discovery and innovation in the biomedical sciences, focusing on cancer, AIDS, and emerging infectious diseases. Position Overview: PROGRAM DESCRIPTION The Basic Science Program (BSP) pursues independent, multidisciplinary research in basic and applied molecular biology, immunology, retrovirology, cancer biology, and human genetics. Research efforts and support are an integral part of the Center for Cancer Research (CCR) at the Frederick National Laboratory for Cancer Research (FNLCR). The Molecular Targets Program (MTP) provides the focus and infrastructure that enables CCR investigators to pursue molecularly targeted drug discovery research by promoting an interdisciplinary, collaborative, team-oriented approach to identifying and validating potential cancer-pertinent targets. The Natural Products Chemistry Section (NPCS) of the MTP, under the scientific direction of Dr. Kirk Gustafson, studies the structural chemistry and biological properties of naturally occurring compounds from terrestrial plants, marine organisms, and microbial isolates. His discovery of biologically active natural products has provided novel structural classes of complex secondary metabolites with unusual molecular scaffolds and functional group arrays. KEY ROLES/RESPONSIBILITIES The NPCS provides research support involving the isolation and identification of novel bioactive compounds with potential therapeutic value for molecular targets of interest to many investigators across CCR. Dedicated research support is needed to provide bioassay-guided isolation and characterization of biologically active small molecules and peptides by spectroscopic (Nuclear Magnetic Resonance [NMR], mass spectrometry, infrared [IR], ultra-violet [UV], optical rotation) and chemical methods. Individual will: Develop chromatographic methods to fractionate natural product extracts and isolate individual metabolites Perform final purification by high performance liquid chromatography (hplc) to provide compounds suitable for spectroscopic analysis Use nmr analysis, liquid chromatography-mass spectrometry (lc-ms), and biological testing methods of chromatography fractions Perform 1-dimension (1d) and 2-dimension (2d) nmr studies and lc-ms characterization of purified natural product compounds Assist in the development of a searchable library of heteronuclear single quantum coherence spectroscopy (hsqc) nmr spectra to aid in dereplication and compound isolation efforts. Assist in the development of ir based dereplication efforts Develop protocols for acquiring ir data on biologically active chromatography fractions and analysis methods Development and implementation of protocols for rapid fractionation of crude natural product extracts Isolation and identification of novel bioactive compounds, data management, and record keeping for all compounds that are isolated Library maintenance for all purified compounds Inventory and re-supply of all necessary equipment and expendables Management, inventory, and service of lab equipment and instruments Individual may oversee the work of college and high school interns Complex assignments are performed independently and reported to supervisor or scientist. May be required to develop new assays or production methods. May supervise a staff of technical personnel. Responsible for data triage and quality control evaluations. Serves as technical authority in the absence of the lead laboratory scientist. Preparation of research plans, proposals, or new production methodologies. May include formal reporting of data including presentation and/or publications. Remain knowledgeable in pertinent areas through literature and scientific presentations. Performs other related duties as assigned by a responsible authority. Function independently in a specialized laboratory of research or specialized scientific service, requiring originality and judgment. Independently evaluate the suitability and quality control of research or production assays or tests; supervise a technical staff. Basic subject matter of specialty area (i.e., biochemistry, immunology, cell biology, etc.); advanced data reporting techniques, mathematics and statistics. Basic laboratory techniques germane to the specialty area (i.e. Sterile procedures, safety procedures, instrument operation, etc.). BASIC QUALIFICATIONS Possession of a Bachelor’s degree from an accredited college/university according to the Council for Higher Education Accreditation (CHEA) in a field related to biomedical research. (Additional qualifying experience may be substituted for the required education). Foreign degrees must be evaluated for U.S. equivalency. In addition to educational requirements, a minimum of eight (8) years related biomedical experience Knowledge and experience in the isolation and structure elucidation of antitumor antimicrobial small molecules and peptides from natural product extracts that are screened in molecularly targeted high-throughput assays Must be able to obtain and maintain a security clearance Equal Opportunity Employer (EOE) | Minority/Female/Disabled/Veteran (M/F/D/V) | Drug Free Workplace (DFW) Title Postdoctoral Fellow Categories Government Organization National Cancer Institute/Center for Cancer Research/Molecular Targets Program Location Frederick, MD Website https://ccr.cancer.gov/molecular-targets-program Contact MTP_PostDoc@nih.gov Job Information The Molecular Targets Program (MTP) of the National Cancer Institute’s Center for Cancer Research (NCI’s CCR) has a postdoctoral position available in its Assay Development and Screening Section (ADSS). The position is for assay development for both primary screening and mechanism of action (MOA) studies on the resulting bioactive compounds obtained from high-throughput screening (HTS) campaigns. The laboratory’s areas of interest include the design and application of HTS assays around newly discovered cancer-related targets and mechanistic hit follow-up with a special emphasis on naturally occurring small molecules. The selected candidate must have demonstrated expertise in utilization of cellular biochemical approaches to assay development or the elucidation of MOA of active compounds derived from phenotypic high-throughput screens. Expertise in one or more of the following areas is desired: cell engineering for assay development, pathway/kinome profiling, high-content imaging assays, expression profiling, protein arrays for binding studies, yeast or mammalian chemical genetic and/or proteomic approaches, and in silico target prediction methods (and follow-up). Candidates with experience with novel assays that can be developed into high throughput screens, or those with the application of CRISPR to the study of elucidation of signaling pathways, are particularly encouraged to apply. The candidates must have a Ph.D. in biochemistry, cell/molecular biology, or a related discipline from an accredited research institution within the last three years. The fellowship duration is 5 years based on performance and yearly evaluations. The fellowship is not restricted to U.S. citizens. The fellowship stipend starts at $54,100 per annum and is determined based on qualifications and years of relevant experience. Applicants should submit a cover letter, CV with bibliography, and three letters of reference to MTP_PostDoc@nih.gov. This position is subject to a background investigation. The NIH is dedicated to building a diverse community in its training and employment programs; underrepresented minority candidates are encouraged to apply. 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